Robust Transfer of Proteins to Membranes Across a Wide Range of pI and Mass Using the Trans-Blot® Turbo System
نویسندگان
چکیده
Bio-Rad’s Trans-Blot Turbo transfer system is based on an improvement of the typical semi-dry blotting approach. The system significantly reduces the blotting time and increases the transfer efficiency over the conventional SD system, giving equivalent or better results compared to the widely used tank blotting process when used with Bio-Rad’s TGX gels. In this study, we tested the performance of the Trans-Blot Turbo transfer system with different gel types; we also compared its performance with tank transfer and Invitrogen’s iBlot system using a rat liver sample separated on 2-D gels to compare a wide range of proteins with different isoelectric points (pI) and masses.
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