Selection of Reference Genes for RT-qPCR Analysis in <em>Coccinella septempunctata</em> to Assess Un-intended Effects of RNAi Transgenic Plants

Corrigendum: Selection of Reference Genes for RT-qPCR Analysis in Coccinella septempunctata to Assess Un-intended Effects of RNAi Transgenic Plants

The development of genetically engineered plants that employ RNA interference (RNAi) to suppress invertebrate pests opens up new avenues for insect control. While this biotechnology shows tremendous promise, the potential for both non-target and off-target impacts, which likely manifest via altered mRNA expression in the exposed organisms, remains a major concern. One powerful tool for the anal...

Genetic transformation of Tomato with three pathogenesis-related protein genes for increased resistance to Fusarium oxysporum f.sp. lycopersici

Fusarium wilt caused by Fusarium oxysporum f.sp. Lycopersici is one of the major obstacles to the production of tomato which causes huge losses in tomato products worldwide. In order to increase the tolerance to this disease, a triple structure containing PR1, chitinase and glucanase genes controlled by 35S promoter was transferred to tomato. Eight days after planting on pre-culture me...

Selection of Reference Genes for RT-qPCR Analysis in a Predatory Biological Control Agent, <em>Coleomegilla maculata</em> (Coleoptera: Coccinellidae)

TRANSGENIC PLANTS & INSECTS Development of an Early-Tier Laboratory Bioassay for Assessing the Impact of Orally-Active Insecticidal Compounds on Larvae of Coccinella septempunctata (Coleoptera: Coccinellidae)

Early-tier studies are the initial step in the environmental risk assessment of genetically engineered plants on nontarget arthropods. They are conducted in the laboratory where surrogate species are exposed to higher concentrations of the arthropod-active compound than those expected to occur in the Þeld. Thus, early-tier tests provide robust data and allow to make general conclusions about th...

Selection of Reference Genes for RT-qPCR Analysis in the Monarch Butterfly, <em>Danaus plexippus</em> (L.), a Migrating Bio-Indicator

Reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) is a powerful technique to quantify gene expression. To facilitate gene expression study and obtain accurate results, normalization relative to stably expressed reference genes is crucial. The monarch butterfly, Danaus plexippus (L.), is one of the most recognized insect species for its spectacular annual migration across No...