Iodine-Mediated Inactivation of Lipid- and Nonlipid-Enveloped Viruses in Human Antithrombin I11 Concentrate

Human plasma-derived protein concentrates intended for clinical use must be treated for viral inactivation to ensure patient safety. This study explored the use of liquid iodine for inactivation of several lipidand nonlipid-enveloped viruses in an antithrombin 111 (AT-Ill) concentrate. Iodine at levels of 0.01% to 0.02% caused between 43% and 94% loss of AT-Ill activity, as well as degradation of AT-Ill as shown by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot analysis. However, addition of up to 0.1% human albumin protected the AT-Ill against