Synthetic In Vitro Transcriptional Oscillators Supplementary Information

نویسندگان

  • Jongmin Kim
  • Erik Winfree
چکیده

1 Model equations 2 1.1 A simple model of the two-switch negative feedback oscillator (Design I) . . . . . . . . . . . . . . . . . 2 1.1.1 Two-dimensional simple models . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 1.2 A simple model of the amplified negative feedback oscillator (Design II) . . . . . . . . . . . . . . . . . 7 1.3 A simple model of the three-switch ring oscillator (Design III) . . . . . . . . . . . . . . . . . . . . . . . 10 1.4 A detailed model of the two-switch negative feedback oscillator (Design I) . . . . . . . . . . . . . . . . 13 1.5 A detailed model of the amplified negative feedback oscillator (Design II) . . . . . . . . . . . . . . . . 15 1.6 A detailed model of the three-switch ring oscillator (Design III) . . . . . . . . . . . . . . . . . . . . . . 16 1.7 Extended model equations including incomplete degradation products . . . . . . . . . . . . . . . . . . 17 1.8 Random sampling technique in the detailed model . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Synthetic in vitro transcriptional oscillators

The construction of synthetic biochemical circuits from simple components illuminates how complex behaviors can arise in chemistry and builds a foundation for future biological technologies. A simplified analog of genetic regulatory networks, in vitro transcriptional circuits, provides a modular platform for the systematic construction of arbitrary circuits and requires only two essential enzym...

متن کامل

Strategy Revealing Phenotypic Differences among Synthetic Oscillator Designs

Considerable progress has been made in identifying and characterizing the component parts of genetic oscillators, which play central roles in all organisms. Nonlinear interaction among components is sufficiently complex that mathematical models are required to elucidate their elusive integrated behavior. Although natural and synthetic oscillators exhibit common architectures, there are numerous...

متن کامل

Research Article: A rapid approach to assess estrogenic transcriptional activity of bisphenol A in the liver of goldfish

Bisphenol A (BPA) is a synthetic compound widely used in types of consumer goods and medical tools. It has been shown that BPA acts as an endocrine disruptor chemical casuing negative impact on reproductive functions. The present study used an in vitro approach to assess estrogenic transcriptional activity of BPA as a rapid and sensitive method. The cultured pieces of hepatic tissue of goldfish...

متن کامل

A novel statistical approach identifies feedback interactions for the construction of robust stochastic transcriptional oscillators

Synthetic biology can be defined as applying engineering approaches, such as part standardisation, abstraction and mathematical modelling, to the design and engineering of novel biological systems. Engineering transcriptional networks presents many challenges including inherent uncertainty in the system structure, changing cellular context and stochasticity in the governing dynamics. One approa...

متن کامل

Load capacity improvements in nucleic acid based systems using partially open feedback control.

Synthetic biology is facilitating novel methods and components to build in vivo and in vitro circuits to better understand and re-engineer biological networks. Recently, Kim and Winfree have synthesized a remarkably elegant network of transcriptional oscillators in vitro using a modular architecture of synthetic gene analogues and a few enzymes that, in turn, could be used to drive a variety of...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2010