Sclero-cornea and defective mesodermal migration.

Sclero-Corneal Trephining

Sclero-Corneal Trephining for Staphyloma

on sclero-corneal trephining, has not yet, I believe, led to the publication of any cases. During 1913 I operated on 17 eyes for this condition, and the results appear to me to be sufficiently encouraging to merit publication. The cases were not selected, my object being first to satisfy myself that reduction of a staphyloma could be effected by establishing free filtration between the aqueous,...

20-P017 Visualizing mesodermal cell migration in chicken embryo

The WAS protein (WASp) coordinates actin polymerization at the leading edge of migrating cells and is defective in patients suffering from Wiskott Aldrich syndrome, resulting in severe immunodeficiency. To model this disease we are using translucent Zebrafish larvae and have begun to characterise a newly derived WASp mutant. The mutant codes for a heavily truncated protein, which we are assayin...

Transplantation studies to investigate mesoderm-ectoderm adhesive cell interactions during gastrulation.

Experiments involving transplantation of the roof of the blastocoel in Rana pipiens embryos reveal that the inner surface of the roof of the blastocoel must be coated with a fibrous extracellular matrix (F-ECM) to serve as a substratum for mesodermal cell migration. When the roof of the blastocoel is inverted the original outer surface, now projecting toward the blastocoel, does not become coat...

An ECM substratum allows mouse mesodermal cells isolated from the primitive streak to exhibit motility similar to that inside the embryo and reveals a deficiency in the T/T mutant cells.

The mesodermal cell layer is created by ingression and migration of the cells from the primitive streak region in mouse embryos on day 7 of pregnancy. In order to study the mechanisms of mesodermal cell migration during development, the mesodermal cells isolated from the primitive streak were cultured on various substrata, and cell behaviour and motility were analysed with a time-lapse video sy...