Improving the Efficiency of Homologous Gene Replacement by Disrupting Non-Homologous End Joining Pathway Gene KusA in the Oleaginous Fungus Mortierella alpina
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چکیده
filamentous fungus, is one of several industrial strains known for the production of arachidonic acid. It is also of particular interest for hydrocarbon biofuel production since it is able to produce up to 50% of its mass in rich, long-chain polyunsaturated fatty acids [PUFA’s]. M. alpina already has mechanisms for accumulating significant concentrations of hydrocarbon compounds, making it a naturally equipped candidate to handle potential toxic concentrations of hydrocarbons. Improving the Efficiency of Homologous Gene Replacement by Disrupting the Non-Homologous End Joining Pathway Gene KusA in Mortierella alpina Kathleen Krueger, Ugur Uzuner, Scott E. Baker, Ziyu Dai
منابع مشابه
Disruption of lig4 improves gene targeting efficiency in the oleaginous fungus Mortierella alpina 1S-4.
The oil-producing zygomycete Mortierella alpina 1S-4 is known to accumulate beneficial polyunsaturated fatty acids. We identified the lig4 gene that encodes for a DNA ligase 4 homolog, which functions to repair double strand breaks by non-homologous end joining. We disrupted the lig4 gene to improve the gene targeting efficiency in M. alpina. The M. alpina 1S-4 Δlig4 strains showed no defect in...
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