Optimizing Recombinant Microbial Fermentation Processes

be established between recombinant and cellular protein biosynthesis, then stable and prolonged recombinant protein production is possible. Extending recombinant protein formation is the key to increased product yields. Five periods of doubling are needed to achieve maximum specific content. The specific content numbers can be derived from the kinetics of the recombinant protein production (4). Host cells, by providing the protein synthesis machinery, building blocks, and energy for the production of recombinant proteins, are critical to the process. Those factors have not always been fully considered in the past.