High - efficiency Scarless Genetic Modification in 2 Escherichia coli Using Lambda - Red Recombination and I - SceI 3 Cleavage 4 5 Method for Bacterial Genetic Modification
نویسندگان
چکیده
Junjie Yang, Bingbing Sun, He Huang, Yu Jiang, Liuyang Diao, Biao Chen, 8 Chongmao Xu, Xin Wang, Jinle Liu, Weihong Jiang, Sheng Yang 9 10 1 Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai 11 Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China 12 2 Shanghai Research Center of Industrial Biotechnology, Shanghai 201201 , China 13 3 University of the Chinese Academy of Sciences, Beijing 100080, China 14
منابع مشابه
Two-step red-mediated recombination for versatile high-efficiency markerless DNA manipulation in Escherichia coli.
Red recombination using PCR-amplified selectable markers is a well-established technique for mutagenesis of large DNA molecules in Escherichia coli. The system has limited efficacy and versatility, however, for markerless modifications including point mutations, deletions, and particularly insertions of longer sequences. Here we describe a procedure that combines Red recombination and cleavage ...
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