Brophy, Peter M. and Ben-Smith, A.W. and Brown, Alan and Behnke, Jerzy M. and Pritchard, D.I. (1995) Differential expression of glutathione S-transferase (GST) by adult Heligmosomoides polygyrus during primary infection in fast and slow

Brophy P. M., Ben-Smith A., Brown A., Behnke J. M. & Pritchard D. I. 1995. Differential expression of glutathione S-transferase (GST) by adult Heligmosomoides polygyrus during primary infection in fast and slow responding hosts. Znternutionaf Journal for Parasitology 25: 641-645. Glutathione S-transferase (GST) specitlc enzymatic activity, assayed with the model substrate l-chloro-2,4dinitrobenzene, was 45% higher in adult Heligmosomoides pofygyrus passaged through a slow responder mouse strain, C57/BLlO compared to worms passaged through a fast-responder strain (SWR X SJL) FI. Western analysis using polyclonal antisera raised to purified H. polygyrus GSTs did not appear to positively correlate the expression of GST protein with functional enzymatic activity. However, western blotting did indicate a sex-linked expression pattern of GST protein, with male worms expressing a higher ratio of the 24 kDa to the 23 kDa GST family than female worms.