Inducing a Strong Th1 Response Mice from Virulent Challenge without Protects Highly Susceptible Leishmania major Vaccination with Phosphoglycan-Deficient

Vaccination with phosphoglycan-deficient Leishmania major protects highly susceptible mice from virulent challenge without inducing a strong Th1 response.

Long-term immunity to Leishmania may require the continued presence of parasites, but previous attempts to create attenuated parasites that persist without causing disease have had limited success. Since Leishmania major mutants that lack lipophosphoglycan and other secreted phosphoglycans, termed lpg2-, persist indefinitely in infected mice without inducing any disease, we tested their ability...

Deficiency of Leishmania Phosphoglycans Influences the Magnitude but Does Not Affect the Quality of Secondary (Memory) Anti-Leishmania Immunity

Despite inducing very low IFN-γ response and highly attenuated in vivo, infection of mice with phosphoglycan (PG) deficient Leishmania major (lpg2-) induces protection against virulent L. major challenge. Here, we show that mice infected with lpg2- L. major generate Leishmania-specific memory T cells. However, in vitro and in vivo proliferation, IL-10 and IFN-γ production by lpg2- induced memor...

Bivalent DNA Vaccination with Genes Encoding Leishmania major Cysteine Proteinases Type I and II Protects Mice Against Infectious Challenge

Western Blot Analysis of Sera from Leishmania major-Infected BALB/c and C57BL/6 Mice

In Leishmania (L.) major-infected BALB/c mice Th2-type cells results in disease progression, whereas C57BL/6-infected mice mount a Th1-type response, which leads to control of the infection. Th2 response correlates with IgG1 whereas, Th1 response supports switching to IgG2a subclasses. Since IgG isotype-dominated response depends on different CD4 + T cell subsets, we studied the antigenic profi...

Evaluation of immune response against autoclaved Leishmania major incorporated into positively charged liposomes in Balb/c mice

Background and aim: Efficacy of vaccines is mainly depending on the type of adjuvant used. Efficacy trials of first generation vaccine against leishmaniasis showed a limited efficacy due to lack of an appropriate adjuvant. The objective of this study was to investigate whether positively charged liposomes containing ALM could induce Th1 type immune response in susceptible Balb/c mice.Mate...