Transmission of cocoa swollen shoot virus by seeds.

نویسندگان

  • A K Quainoo
  • A C Wetten
  • J Allainguillaume
چکیده

A study was undertaken to determine whether cocoa swollen shoot virus is transmitted by seeds, to improve the robustness of quarantine procedures for international exchange and long term conservation of cocoa germplasm. PCR/capillary electrophoresis, using cocoa swollen shoot virus primers designed from the most conserved regions of the six published cocoa genome sequences, allowed the detection of cocoa swollen shoot virus in all the component parts of cocoa seeds from cocoa swollen shoot virus-infected trees. PCR/capillary electrophoresis revealed the presence of cocoa swollen shoot virus in seedlings raised from seeds obtained from cocoa swollen shoot virus-infected trees. The high frequency with which the virus was transmitted through the seedlings suggested that cocoa swollen shoot virus is transmitted by seeds. This has serious implications for cocoa germplasm conservation and distribution.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Early signs of infection in Cacao swollen shoot virus (CSSV) inoculated cocoa seeds and the discovery of the cotyledons of the resultant plants as rich sources of CSSV

Despite the huge efforts and funds expended to try and bring Cacao swollen shoot virus (CSSV) disease under control, the disease has remained largely intractable. Inspired by the need to inject new ideas into the current breeding programmes for CSSV resistance, the interactions between cocoa and the virus in CSSV-infected plants from 3 days post inoculation (dpi) to about 30 dpi were studied us...

متن کامل

The effectiveness of somatic embryogenesis in eliminating the cocoa swollen shoot virus from infected cocoa trees.

Investigations were undertaken on the use of somatic embryogenesis to generate cocoa swollen shoot virus (CSSV) disease free clonal propagules from infected trees. Polymerase chain reaction (PCR) capillary electrophoresis revealed the presence of CSSV in all the callus tissues induced from the CSSV-infected Amelonado cocoa trees (T1, T2 and T4). The virus was transmitted to primary somatic embr...

متن کامل

- Forum - Control of Plant Virus Diseases in Sub-saharan Africa: the Possibility and Feasibility of an Integrated Approach

Plant viruses and virus diseases have been studied for more than 100 years and much attention has been given to their control. However, this has been difficult to achieve because of the lack of any effective means of curing virus-infected plants. Chemotherapy, thermotherapy and meristem-tip culture can be successful, but they cannot be used on a large scale. Consequently, the main approach has ...

متن کامل

Role of Roasting on the Sensory and Physicochemical Properties of Cocoa Fudge

Cocoa seeds were roasted at 120oC for 10 and 20 minutes were ground to a fine paste to obtain cocoa liquor. Cocoa fudge samples were prepared using the prepared liquor cocoa butter, sugar and lecithin. The two samples were analyzed for their physicochemical and sensory properties. The results of the study reveal that the roasting duration has a significant influence on both the properties. An i...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Journal of virological methods

دوره 150 1-2  شماره 

صفحات  -

تاریخ انتشار 2008