Proanthocyanidins from Grape Seeds Modulate the NF-κB Signal Transduction Pathways in Rats with TNBS-Induced Ulcerative Colitis.

نویسندگان

  • Xiaoli Li
  • Xiaolai Yang
  • Yongqing Cai
  • Hong Qin
  • Li Wang
  • Yanhong Wang
  • Yanhui Huang
  • Xiaoxia Wang
  • Shuai Yan
  • Liping Wang
  • Xin Zhao
  • Wan Li
  • Sijia Li
  • Jiajia Chen
  • Yongjie Wu
چکیده

To elucidate the molecular mechanisms involved in the therapeutic effects of proanthocyanidins from grape seeds (GSPE), we explore whether GSPE regulates the inflammatory response of TNBS-induced colitis in rats at the levels of NF-κB signal transduction pathway. Rats were intragastrically administered of different doses of GSPE (100, 200 and 400 mg·kg-1) per day for seven days after ulcerative colitis (UC) was induced by intracolonic injection of 2,4,6-trinitrobenzenesulfonic acid (TNBS) dissolved in 50% ethanol. Sulfasalazine (SASP) at 400 mg/kg was used as a positive control drug. The expression of nuclear factor-kappa B (NF-κB), phospho-I kappaB-alpha (pIκBα), inhibitor kappa B kinase (IκK) in the colon tissues were all measured by enzyme-linked immunosorbent assay (ELISA) methods. Treatment with GSPE reduced the expression of NF-κB, pIκBα and IκK in the colon. The results of this study show that GSPE exerts beneficial effects in inflammatory bowel disease by inhibition of NF-κB signal transduction pathways.

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عنوان ژورنال:
  • Molecules

دوره 16 8  شماره 

صفحات  -

تاریخ انتشار 2011