Reductive cleavage of disulfide bridges in ribonuclease.
نویسندگان
چکیده
منابع مشابه
Synthesis of Zinc Dimethyldithiocarbamate by Reductive Disulfide Bond Cleavage of Tetramethylthiuram Disulfide in Presence of Zn2+
The zinc(II) complex [Zn2(dmdtc)2(μ-dmdtc)2] has been synthesized directly from thiram ligand, containing a disulfide bond {dmdtc = N,N-dimethyldithiocarbamate; thiram = N,N-tetramethylthiuram disulfide}, and characterized by elemental analysis and spectroscopic methods. Surprisingly thiram, undergoes a reductive disulfide bond scission upon reaction with Zn2+ in methanolic media to give the [Z...
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Disulfide bonds are undoubtedly of great importance in stabilizing the native conformations of many proteins. For studies of the covalent structure of proteins it is desirable, as a first step, to cleave the disulfide bonds (1). This has been carried out either by oxidation (l-3) or by reduction followed by alkylation of the sulfhydryl groups formed (4). Iodoacetic acid (or iodoacetamide) has b...
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The RNase molecule consists of a single chain, arranged in a compact, folded structure, cross-linked through 4 disulfide bridges (1). After hydrolysis to determine the amino acid composition of RNase, 8 half-cystine or cysteic acid residues have been identified (2,3) and the approximate location of these residues in the partial structural formula for oxidized RNase has been determined (4). More...
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In studies on the chemical structure of ribonuclease in which trypsin is used as a specific reagent for hydrolysis (2), it has been found necessary first to cleave the disulfide bridges in the molecule by oxidation with performic acid in a manner similar to that employed by Sanger (3) in his investigation of insulin. Oxidation is required in order to render the protein more readily susceptible ...
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Disulfide bonds between the side chains of cysteine residues are the only common crosslinks in proteins. Bovine pancreatic ribonuclease A (RNase A) is a 124-residue enzyme that contains four interweaving disulfide bonds (Cys26-Cys84, Cys40-Cys95, Cys58-Cys110, and Cys65-Cys72) and catalyzes the cleavage of RNA. The contribution of each disulfide bond to the conformational stability and catalyti...
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ورودعنوان ژورنال:
- Science
دوره 125 3250 شماره
صفحات -
تاریخ انتشار 1957