A simple method for desalting biological fluids for paper chromatography of amino-acids.

نویسندگان

  • N VERGHESE
  • P N RAMAKRISHNAN
چکیده

A quick method of performing the Paul-Bunnell reaction is described which is as sensitive as the orthodox method. When read at two hours the rapid test appears to be definitely more sensitive than the orthodox method. When the rapid method is performed in the later stages of glandular fever a much higher titre is obtained than by the orthodox method. This might prove to be of diagnostic importance in such cases, particularly if they have not been investigated in the early stages. The validity of this point is being investigated further.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Simple Method for Desalting Biological Fluids for Paper Chromatography of Amino-acids

A quick method of performing the Paul-Bunnell reaction is described which is as sensitive as the orthodox method. When read at two hours the rapid test appears to be definitely more sensitive than the orthodox method. When the rapid method is performed in the later stages of glandular fever a much higher titre is obtained than by the orthodox method. This might prove to be of diagnostic importa...

متن کامل

Paper chromatography of free amino acids in human blood serum.

BOTJLANGER and BISERrE (1) used a solution of acid acetone for the extraction of amino acids in biologic material. The separation of amino acids on buffered papers described by Ryb#{225}r, Touek, and Hais, (2) and Hais and Hordovs/q (3) used the combination of tertiary butanolborate buffer system (pH 8.5) for separation of amino acids on paper. In our laboratories we have developed a chromatogr...

متن کامل

Two-dimensional paper chromatographic determination of amino acids in urine.

DENT (1) introduced paper chromatography for tile ilivestigatioll of amino-aciduria in 1948. However, his one-dimensional system yielded inadequate separation, and his two-dimensional method required 3 days. Since then, technics utilizing gas chromatography (2-4), thin layer chromatography, column chromatography, and high voltage electrophoresis (5-7) have been published for tile determination ...

متن کامل

Effective Solvent Systems for Separation of PTH-Amino Acids on Polyamide Sheet

Two dimensional thin layer chromatography on polyamide sheets is a useful method for identification of PTH-amino acids which have very close Rf-values. The solvent systems described here are particularly effective and have an edge over other solvent systems used for the separation of PTH-amino acids. The solvent systems provide a clear separation of all PTH-amono acids except lle / Leu in a...

متن کامل

Two-way separation of amino acids and other ninhydrin-reacting substances by high-voltage electrophoresis followed by paper chromatography.

One-way separation of amino acids by electrophoresis at high voltage is now a well-established procedure (Gross, 1955). With the large number of amino acids often found in biological fluids, it is, however, very difficult to obtain satisfactory separation without overlap of spots. Several investigators have used techniques for the separation of amino acids and other substances by a combination ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Journal of clinical pathology

دوره 10 1  شماره 

صفحات  -

تاریخ انتشار 1957