Preparation of stable sensitized erythrocytes for detection of chlamydial antibodies.
نویسندگان
چکیده
Sheep erythrocytes were treated with glutaraldehyde before sensitization for the indirect hemagglutination test to assay chlamydial antibodies. This treatment markedly increased stability during storage.
منابع مشابه
Indirect hemagglutination test for chlamydial antibodies.
An indirect hemagglutination (IHA) test is described for chlamydial antibodies in psittacosis diagnostic sera; for this test tanned sheep erythrocytes sensitized with a deoxycholate extract of Chlamydia psittaci grown in Vero cell monolayers were used. Adaptation of the IHA test to the Microtiter system decreased sensitivity; nevertheless, the Microtiter-IHA test was more sensitive than the com...
متن کاملUse of stable, sensitized cells in an indirect microhemagglutination test for melioidosis.
Two evaluations were carried out in this study. The first was a comparison of the standard tube test with the automated microtitration test for the detection of antibodies to Pseudomonas pseudomallei by the indirect hemagglutination method. Data from this comparison indicated that the tests were equivalent. The second evaluation consisted of reproducibility studies on two lots of pyruvic aldehy...
متن کاملGrowth of Leptospires and Antigen Extraction
SYNOPSIS Sheep red cells, sensitized with a genus-specific leptospiral substance extracted from a water leptospira, were preserved by freeze drying. The serological reactivity of the preparation was tested against a total of 41 sera from proven cases of human leptospirosis and 27 from proven cases of animal leptospirosis. The sensitized, freeze-dried red cells appeared to be a sensitive reagent...
متن کاملThe Effects of Recombinant Human Erythropoietin (rHuEPO) on Antihuman Lymphocyte Antibodies Titer in Sensitized Rats
Erythropoietin (EPO) was first known as a factor for red blood cell proliferation and differentiation. Recent studies show the effects of EPO on immune system. In this study the antihuman leukocyte antibody (anti-HLA) titer were determined in five groups of rats, which had been sensitized with human lymphocyte. Also, the effects of stimulation's frequency and dose of recombinant human erythropo...
متن کاملDetection of chlamydial infection in Iranian turkey flocks
Background: Avian chlamydiosis is a zoonotic disease ofbirds caused by the intracellular bacterium Chlamydia psittaci. Avianchlamydiosis leads to severe respiratory disease in young turkeys and eggproduction losses in layers. OBJECTIVES: Due to paucity of information about theprevalence of chlamydial infection in the turkey population in Iran, this studywas conducted to detect chlamydial infect...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Journal of clinical microbiology
دوره 1 1 شماره
صفحات -
تاریخ انتشار 1975