SIMULTANEOUS ESTIMATION OF ABACAVIR, LAMIVUDINE AND ZIDOVUDINE IN COMBINED TABLET DOSAGE FORM BY UV SPECTROPHOTOMETRIC METHOD Nagulwar Vaishali P* and Bhusari Kishor P

The present study deals with the development of an accurate, economical and reproducible UV spectrophotometric method for the simultaneous estimation of abacavir, lamivudine and zidovudine in pure bulk drug and in combined tablet dosage form. The stock solutions were prepared in acetonitrile followed by the further required dilutions with distilled water. The λ max for abacavir, lamivudine, and zidovudine were observed at 295.6, 279.8 and 266.2 nm respectively and linearity was also shown at these wavelengths in concentration range of 5-30 μg/ mL, 5-25μg/ mL and 5-30μg/mL by all the three drugs. Mixtures containing abacavir -30 μg/mL, lamivudine-15μg/mL and zidovudine30μg/mL were analyzed at their respective wavelengths and using the simultaneous equations (Cramer’s rule), their contents were calculated. The proposed method has estimated abacavir 99.25%, lamivudine 99.12% and zidovudine 100.16% in standard mixture and 98.55%, 99.70 and 99.92% respectively in the marketed tablets. The results of analysis have been validated statistically and also by recovery studies. Thus the present study gives an excellent method for the determination of all the three drugs in combined dosage formulation without their prior separation.