Pii: S0887-2333(00)00010-2
نویسندگان
چکیده
ÐWe have established a new test concept for in vitro pharmacological trials. Our model employs tissue explants to test compounds for toxicity which arises with the metabolic interactions among dierent cell types. Microsurgical preparation of tissue explants avoids the destruction of the organ-speci®c tissue architecture. Explants were mounted in tissue carriers to improve nutrition and handling of the sample. To allow for the omission of serum supplementation of the culture medium, explants were cultured under continuous medium ̄ow. Test substances are applied considering the polar architecture of most tissues in vivo, for example, covering the apical aspect of epithelia. In principal, all tissues obtained from any species, including man, can be used in this system. A trial application was performed with vitreous body substitutes, substances used in ophthalmology. One compound had passed cell culture tests, but caused massive blood vessel deterioration in vivo. Using our test system based on the developing renal vessel system, we could con®rm, within 24 hours, severe vessel damage which resembles the injury suered by the rabbit retina. We demonstrate that an improved tissue culture assay is a suitable tool for the detection of toxicity that remained unidenti®ed in cell culture tests. # 2000 Elsevier Science Ltd. All rights reserved
منابع مشابه
Pii: S0925-7721(00)00010-9
We improve previous lower bounds on the number of simple polygonizations, and other kinds of crossing-free subgraphs, of a set of N points in the plane by analyzing a suitable configuration. We also prove that the number of crossing-free perfect matchings and spanning trees is minimum when the points are in convex position. 2000 Elsevier Science B.V. All rights reserved.
متن کاملPii: S0887-2333(98)00048-4
ÐThe ototoxic damage that drugs such as neomycin, kanamycin, colistin, cisplatin, transplatin and carboplatin cause on outer and inner hair cells in postnatal day 3 rat cochlear explants was investigated. Phalloidin± ̄uorescein conjugate-stained stereocilia bundles of sensory hair cells were quanti®ed by video image analysis as a measurement of ototoxic eect. The video image quanti®cation syste...
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