Production of poly-b-hydroxybutyrateon molasses by recombinantEscherichia coli

نویسندگان

  • Fang Liu
  • Wenqing Li
  • Darin Ridgway
  • Tingyue Gu
  • Zhongyao Shen
چکیده

Beet molasses successfully replaced glucose as sole carbon source to produce poly-b-hydroxybutyrate by a recombinant Escherichia coli strain (HMS174/pTZ18u-PHB). The fermentation with molasses was cheaper than with glucose. The final dry cell weight, PHB content and PHB productivity were 39.5 g/L, 80% (w/w) and 1 g/Lh, respectively, in a 5 L stirred tank fermenter after 31.5 h fed-batch fermentation with constant pH and dissolved O2 content. Biotechnology Letters, Vol 20, No 4, April 1998, pp. 345–348

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Economical production of poly-3-hydroxybutyrate by Bacillus cereusunder submerged and solid state fermentation

High cost of poly-3-hydroxybutyrate production remained a major hindrance for its wide range applications. In the current study poly-3-hydroxybutyrate producing bacteria were isolated from environmental sources. Highest poly-3hydroxybutyrate producing isolate Bacillus cereus PS 10 was investigated for its ability to use wide range of low-cost carbon sources including agro-industrial residues, v...

متن کامل

Production of Polyhydroxyalkanoates (PHAs) By Bacillus subtilis and Escherichia coli Grown on Cane Molasses Fortified with Ethanol

The aim of this work was to study the production of polyhydroxyalkanoates (PHAs) by Bacillus subtilis and Escherichia coli isolated from the industrial contaminated soil samples using cane molasses as an inexpensive substrate. The amount of PHA accumulated followed a similar pattern to its growth for each of treatment indicating a growth-related production, yielding maximum PHA production of 54...

متن کامل

Isolation and Identification of Poly β-Hydroxybutyrate Over-Producing Bacteria and Optimization of Production Medium

BACKGROUND Biodegradable polyesters are candidates for the development of environmental friendly plastics. Poly-β-hydroxybutyrate (PHB) is a type of polyester from the hydroxyalkanoates family, synthesized by bacteria as an intracellular material and accumulated as granules in the cytoplasm. OBJECTIVES The aim of this study was to isolate Poly β-hydroxybutyrate over producing bacteria and opt...

متن کامل

Production of polyhydroxyalkanoates in sucrose-utilizing recombinant Escherichia coli and Klebsiella strains.

The cloned poly-3-hydroxybutyrate (PHB) synthesis pathway from Alcaligenes eutrophus has been introduced into sucrose-utilizing strains of Escherichia coli, Klebsiella aerogenes, and Klebsiella oxytoca. The plasmid-borne genes were well expressed in these environments and were able to mediate the production of significant amounts of PHB when the bacteria were grown with sucrose as the sole carb...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 1998