Characterization of the ceramide moieties of sphingoglycolipids from mouse brain by electrospray-tandem mass spectrometry: identification of ceramides containing sphingadienine
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چکیده
Sphingoglycolipids (SGL) are cell membrane constituents. As the ceramide structure influences the biological properties of the SGL, we characterized by electrospray tandem mass spectrometry the molecular species of ceramides present in SGL of mouse brain. We report here for the first time the presence in mammalian brain of sphingadienine (d18:2). Sphingenine (d18:1) is present in all SGL species in contrast to eicosasphingenine (d20:1) which is only a constituent of gangliosides. Sphingadienine is present in galactosylceramide and sulfatides. Free ceramides contain the three types of bases. Thus there could be two separate pools of free ceramides (d18:1; d18:2) and (d20:1; d18:1) as precursors of complex
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Characterization of the ceramide moieties of sphingoglycolipids from mouse brain by ESI-MS/MS: identification of ceramides containing sphingadienine.
Sphingoglycolipids (SGLs) are cell membrane constituents. As the ceramide structure influences the biological properties of the SGL, we characterized by electrospray ionization tandem mass spectrometry the molecular species of ceramides present in SGL of mouse brain. We report here for the first time the presence in mammalian brain of sphingadienine (d18:2). Sphingenine (d18:1) is present in al...
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تاریخ انتشار 2003