University of Oklahoma Graduate College Metagenomic Insights into Microbial Community Responses to Long-term Elevated Co2 a Dissertation
نویسندگان
چکیده
......................................................................................................................... xvi Chapter 1: Introduction ..................................................................................................... 1 1.1 Atmospheric CO2: the background ....................................................................... 1 1.2 Effects of elevated atmospheric CO2 on macroecosystems ................................. 1 1.3 Effects of elevated atmospheric CO2 on soil microbial communities .................. 3 1.4 Microbial biodiversity and current challenges ..................................................... 6 1.5 Foci of this study .................................................................................................. 9 Chapter 2: Strain/Species Identification in Metagenomes Using Genome-Specific Markers ............................................................................................................... 13 2.1 Abstract ............................................................................................................... 13 2.2 Introduction ........................................................................................................ 15 2.3 Materials and Methods ....................................................................................... 18 2.3.1 Data resources ........................................................................................... 18 2.3.2 Selection of genome-specific markers (GSMs) ......................................... 19 2.3.3 Specificity evaluation of GSMs ................................................................. 21 2.3.4 Determining the detection limit and true positive thresholds .................... 22 2.3.5 Profiling T2D-/obesity-associated microbial strains ................................. 22 2.4 Results ................................................................................................................ 23
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