Four-part Differential Leukocyte Count Using Flow Cytometer
نویسندگان
چکیده
This paper reports the four-part differential leukocyte count (DLC) of human blood using a MEMS microflow (flow) cytometer. It is achieved with a two-color laser-induced fluorescence (LIF) detection scheme. Four types of leukocytes including neutrophils, eosinophils, lymphocytes and monocytes are identified in blood samples, which are stained by fluorescein isothiocyanate (FITC) and propidium iodide (PI). The DLC results show good correlation with the count from a commercial hematology analyzer. The whole system is also implemented into a portable instrument for space application.
منابع مشابه
Four-part leukocyte differential count based on sheathless microflow cytometer and fluorescent dye assay.
Leukocyte differential count is one of the most frequently ordered clinical tests in hospitals. This paper reports a point-of-care test for the leukocyte count by using a microflow cytometer and a fluorescent dye assay. The dye assay relied on fluorescent detection alone to count leukocytes in blood and to identify leukocyte subtypes. By combining the fluorescent assay with a sheathless microfl...
متن کاملComparison of Venous and Capillary Differential Leukocyte Counts Using a Standard Hematology Analyzer and a Novel Microfluidic Impedance Cytometer
Capillary blood sampling has been identified as a potentially suitable technique for use in diagnostic testing of the full blood count (FBC) at the point-of-care (POC), for which a recent need has been highlighted. In this study we assess the accuracy of capillary blood counts and evaluate the potential of a miniaturized cytometer developed for POC testing. Differential leukocyte counts in the ...
متن کاملDifferential leukocyte count method for bovine low somatic cell count milk.
Whereas many differential leukocyte count methods for high somatic cell count (SCC) milk from mastitic cows are available, only a few have been developed for low SCC milk. We have developed a flow cytometric differential leukocyte count method for low SCC milk. The procedure consists of 1) 1.5 ml of diluted milk sample (30%, vol/vol dilution with PBS), 2) centrifugation, 3) leukocyte labeling w...
متن کاملRapid, effective and user-friendly immunophenotyping of canine lymphoma using a personal flow cytometer
BACKGROUND Widespread use of flow cytometry for immunophenotyping in clinical veterinary medicine is limited by cost and requirement for considerable laboratory space, staff time, and expertise. The Guava EasyCyte Plus (Guava Technologies, Hayward, CA, US) is the first, personal, bench-top flow cytometer designed to address these limitations. OBJECTIVE The aim of this study was to adapt the i...
متن کاملUnsatisfactory performance of flow cytometer UF-100 and urine strips in predicting outcome of urine cultures.
UF-100 flow cytometer and urine strip results were cross-interpreted to predict culture outcomes. The best negative predictive value was obtained with bacteria at > or =1,000/microl, white blood cells at > or =20/microl, or leukocyte esterase positivity. Nine of 24 false negatives were clinically significant. Thus, UF-100 and urine strip results do not accurately predict the outcome of cultures.
متن کامل