Running title: CATALYTIC EFFICIENCY OF ENDO-1,4-β-XYLANASE Probing the Role of Sigma-π Interaction and its Energetics for Catalytic Efficiency of Endo-1,4-β-Xylanase
نویسندگان
چکیده
*Corresponding author. Mailing address: Department of Chemical Engineering, Konkuk University, 1 Hwayang-dong, Seoul 143-701, South Korea. Phone: 82-2-450-3505. Fax: 82-2-458-3504. E-mail: [email protected]. Department of Chemical Engineering, Texas A&M University, College Station TX77845, USA. Phone: 979-862-1610. Fax: 979-845-6446. E-mail: [email protected] Copyright © 2012, American Society for Microbiology. All Rights Reserved. Appl. Environ. Microbiol. doi:10.1128/AEM.02261-12 AEM Accepts, published online ahead of print on 28 September 2012
منابع مشابه
Properties of selected hemicellulases of a multi-enzymatic system from Penicillium funiculosum.
A multi-enzymatic system from Penicillium funiculosum displayed alpha-L-arabinofuranosidase, endo-1,4-beta-D-xylanase, beta-D-xylosidase and endo-1,3-1,4-beta-D-glucanase activities at high levels over a wide acidic pH range of 2.0 to 5.5. Moreover, the pH stability was particularly extended over the wide range of pH of 2.0 to 8.0 with endo-1,3-1,4-beta-D-glucanase and endo-1,4-beta-D-xylanase;...
متن کاملPartial Optimization of Endo-1, 4-Β-Xylanase Production by Aureobasidium pullulansUsing Agro-Industrial Residues
Objective(s): Although bacteria and molds are the pioneering microorganisms for production of many enzymes, yet yeasts provide safe and reliable sources of enzymes with applications in food and feed. Materials and Methods: Single xylanase producer yeast was isolated from plant residues based on formation of transparent halo zones on xylan agar plates. The isolate showed much ...
متن کاملSimultaneous Silencing of Xylanase Genes in Botrytis cinerea
The endo-β-1,4-xylanase BcXyn11A is one of several plant cell-wall degrading enzymes that the phytopathogenic fungus Botrytis cinerea secretes during interaction with its hosts. In addition to its enzymatic activity, this protein also acts as an elicitor of the defense response in plants and has been identified as a virulence factor. In the present work, other four endoxylanase coding genes (Bc...
متن کاملStructural and functional characterization of a highly stable endo-β-1,4-xylanase from Fusarium oxysporum and its development as an efficient immobilized biocatalyst
BACKGROUND Replacing fossil fuel with renewable sources such as lignocellulosic biomass is currently a promising alternative for obtaining biofuel and for fighting against the consequences of climate change. However, the recalcitrant structure of lignocellulosic biomass residues constitutes a major limitation for its widespread use in industry. The efficient hydrolysis of lignocellulosic materi...
متن کاملComputational design of an endo-1,4-beta-xylanase ligand binding site.
The field of computational protein design has experienced important recent success. However, the de novo computational design of high-affinity protein-ligand interfaces is still largely an open challenge. Using the Rosetta program, we attempted the in silico design of a high-affinity protein interface to a small peptide ligand. We chose the thermophilic endo-1,4-β-xylanase from Nonomuraea flexu...
متن کامل