Studies on Newcastle disease virus by the plaque method.

Plaque formation of LaSota pathogenic strain of Newcastle disease virus adapted in chick embryo fibroblast cells

In order to adapt LaSota strain of Newcastle disease virus (NDV) on chick embryo fibroblast (CEF) cells, 0.1 ml from LaSota vaccine produced in Razi vaccine and serum research institute inoculated to the CEF cells and was passaged five times in the CEF grown in Eagle’s minimum essential medium (MEM). First to third passages were blind but in fourth passage cytopathic effect of virus was observe...

Studies on the immunogenic properties of three lentogenic strains of newcastle disease virus

Sensitivity of the plaque technique for the study of selected vaccine strains and a virulent strain of Newcastle disease virus.

A plaque technique was found useful for the differentiation and titration of two vaccine strains and a local highly virulent field isolate of Newcastle disease virus.

Differentiation of Virulent and Non-Virulent Newcastle Disease Virus Isolates Using RT-PCR

Newcastle disease is one of the main concerns of poultry farmers. Detection of virulent strains of Newcastle disease virus (NDV) has a great impact on control measures against the disease. In this study RT-PCR was optimized in high sensitivity in order to differentiate the virulent from non-virulent NDV isolates directly in tissue homogenates. The vaccinal NDV strain and known field isolates we...

Effect of Zataria multiflora essential oil on immune responses and faecal virus shedding period in broilers immunized with live Newcastle disease vaccines

Zataria multiflora essential oil possesses immunomodulatory effects and the present study investigatesits effect on immune responses to live Newcastle disease (ND) vaccines and faecal shedding period ofvaccine strain virus in chickens. One hundred and eighty chickens from both sexes were divided into 6 equalgroups: group 1: unvaccinated, group 2: vaccinated (B1 vaccine followed by La Sota 10 da...