Alginic acid metabolism in bacteria. II. The enzymatic reduction of 4-deoxy-L-erythro-5-hexoseulose uronic acid to 2-keto-3-deoxy-D-gluconic acid.

Alginase, partially purified from cell-free extracts of an Enzyme Purifiation adapted Pseudomonas, has been shown to metabolize alginic All operations were carried out in a cold room at O-3”. The acid with the resultant accumulation of the monosaccharide, preparation of the crude extract of alginic acid-grown Pseudo4-deoxy-r,-erythro-5-hexoseulose uranic acid (ketodeoxygluconalmmae has been described (1). dehyde) (1). The subsequent metabolic steps in the complete Streptomycin Sul&te Fractiolaation-To 28 ml of the crude utilization of this compound have now been elucidated and are extract, 18 ml of a 5% solution of streptomycin sulfate were illustrated in Scheme 1. added slowly and with continuous stirring. After 15 minutes