Fate and stability of 14C-labeled 2,4,6-trinitrotoluene in contaminated soil following microbial bioremediation processes.

Screening Effective Factors in Slurry Phase Bioremediation of 2,4,6-Trinitrotoluene (TNT) Contaminated Soil

Bioremediation of 2,4,6-trinitrotoluene contaminated soil in slurry and column reactors.

The bioremediation of 2,4,6-trinitrotoluene (TNT) contaminated soil was performed on a laboratory scale. To compare bioremediation methods, a soil slurry reactor and a soil column reactor were operated and the effects of supplemental sources were investigated. Optimal conditions for the two bioremediation systems for the removal of TNT were obtained. In the soil slurry reactor, about 60% of the...

Fate and metabolism of [15N]2,4,6-trinitrotoluene in soil.

The fates of the labels from [14C] and [15N] trinitrotoluene were analyzed in bioreactors under aerobic conditions in soil treated by a fungal bioremediation process with Stropharia rugosoannulata and in control soil. Up to 17.5% of the 15N label had a different fate than the 14C label. Three N-mineralization processes were identified in detailed experiments with [15N]TNT. About 2% of the 15N l...

Bioremediation of 2,4,6-trinitrotoluene by bacterial nitroreductase expressing transgenic aspen.

Trees belonging to the genus Populus are often used for phytoremediation due to their deep root formation, fast growth and high transpiration rates. Here, we study the capacity of transgenic hybrid aspen (Populus tremula x tremuloides var. Etropole) which expresses the bacterial nitroreductase gene, pnrA, to tolerate and take-up greater amounts of the toxic and recalcitrant explosive, 2,4,6-tri...

Biodegradation of TNT (2,4,6-trinitrotoluene) by Phanerochaete chrysosporium.

Extensive biodegradation of TNT (2,4,6-trinitrotoluene) by the white rot fungus Phanerochaete chrysosporium was observed. At an initial concentration of 1.3 mg/liter, 35.4 +/- 3.6% of the [14C]TNT was degraded to 14CO2 in 18 days. The addition of glucose 12 days after the addition of TNT did not stimulate mineralization, and, after 18 days of incubation with TNT only, about 3.3% of the initial ...