Spindle Assembly: Kinesin-5 Is in Control
نویسنده
چکیده
Kinesin-5 is essential in many species for the formation of a bipolar spindle. Although bipolar tetramers were known to crossbridge pairs of microtubules, the mechanism for organizing spindles was unclear. However, new experiments have revealed unique properties of kinesin-5, including some associated with the tail domain, that provide clues as to how spindles are assembled.
منابع مشابه
Kinesin-5-independent mitotic spindle assembly requires the antiparallel microtubule crosslinker Ase1 in fission yeast
Bipolar spindle assembly requires a balance of forces where kinesin-5 produces outward pushing forces to antagonize the inward pulling forces from kinesin-14 or dynein. Accordingly, Kinesin-5 inactivation results in force imbalance leading to monopolar spindle and chromosome segregation failure. In fission yeast, force balance is restored when both kinesin-5 Cut7 and kinesin-14 Pkl1 are deleted...
متن کاملA microtubule polymerase cooperates with the kinesin-6 motor and a microtubule cross-linker to promote bipolar spindle assembly in the absence of kinesin-5 and kinesin-14 in fission yeast
Accurate chromosome segregation relies on the bipolar mitotic spindle. In many eukaryotes, spindle formation is driven by the plus-end-directed motor kinesin-5 that generates outward force to establish spindle bipolarity. Its inhibition leads to the emergence of monopolar spindles with mitotic arrest. Intriguingly, simultaneous inactivation of the minus-end-directed motor kinesin-14 restores sp...
متن کاملKinesin-5 inhibitor resistance is driven by kinesin-12
The microtubule (MT) cytoskeleton bipolarizes at the onset of mitosis to form the spindle. In animal cells, the kinesin-5 Eg5 primarily drives this reorganization by actively sliding MTs apart. Its primacy during spindle assembly renders Eg5 essential for mitotic progression, demonstrated by the lethal effects of kinesin-5/Eg5 inhibitors (K5Is) administered in cell culture. However, cultured ce...
متن کاملKinesin-14 and kinesin-5 antagonistically regulate microtubule nucleation by γ-TuRC in yeast and human cells
Bipolar spindle assembly is a critical control point for initiation of mitosis through nucleation and organization of spindle microtubules and is regulated by kinesin-like proteins. In fission yeast, the kinesin-14 Pkl1 binds the γ-tubulin ring complex (γ-TuRC) microtubule-organizing centre at spindle poles and can alter its structure and function. Here we show that kinesin-14 blocks microtubul...
متن کاملKinesin-12 Differentially Affects Spindle Assembly Depending on Its Microtubule Substrate
BACKGROUND During mitosis, the microtubule (MT) cytoskeleton rearranges into a bipolar spindle that drives chromosome segregation. Two kinesin subtypes, kinesin-5 and kinesin-12, help build this bipolar array by separating the spindle poles. However, unlike kinesin-5, the kinesin-12 mechanism is not well understood. RESULTS At physiologically normal protein levels, we demonstrate that the hum...
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ورودعنوان ژورنال:
- Current Biology
دوره 18 شماره
صفحات -
تاریخ انتشار 2008