TITLE Differential activation of astrocytes and microglia after spinal cord injury in the fetal rat

Background: Because the immature spinal cord was nerve growth permissive, we examined glial reactions that influence regeneration of the spinal cord in a fetal rat spinal cord injury model. Methods: Three, 7, 21, and 35 days after intrauterine surgery, offsprings were killed and the thoracic and lumbar spinal cords were carefully removed from the spinal column and then cut into 10 μm longitudinal sections. These sections were stained with hematoxylin-eosin, anti-glial fibrillary acidic protein antibody (GFAP) as a marker of astrocytes, and anti-complement CR3 antibody (OX-42) as a marker of microglia. A cordotomy model in young adult rat was utilized as a control. Results: In the present study, collagen fibers and scar formation were seen in the severed spinal cords of mature rats, but scar formation was not seen in the fetal rat cordotomy group, regardless of spinal continuity. In the Control group, biological activity of GFAP-positive cells increased over time. In the fetal rat cordotomy model, activity elevated slightly immediately after cordotomy, and disappeared shortly thereafter. In the Control group, OX-42 positive macrophage-like cells proliferated over time. However, in the fetal rat cordotomy model, OX-42 positive macrophage-like cells were recognized on postoperative days 3 and 7, and then disappeared. At 5 mm from the cordotomy site, reactive microglia were recognized in the white matter of Control group spinal cords, but these microglia were not