Optimization of Nutritional Constitute for Enhanced Alpha amylase Production Using by Solid State Fermentation Technology

Microbial Alpha amylase is highly demanded industrial enzyme in various sectors such as pharmaceuticals, food, textile and detergents, etc. In this study optimized alpha amylase Production by a strain of Bacillus Subtilis MAFE 118079 in solid state fermentation (SSF) was initiated and Rice bran was used substrate for alpha amylase production. Linear multiple regression analysis showed that maximum amount of amylase production is 1457.7 U/g in 48 h. process optimized enzyme titre, which was maximum (2311.1 U/g) with pH:7 when SSF was carried out at 37°C for 48 h using a substrate with 75% initial moister. Supplementation different carbon sources the soluble starch and glucose enhanced enzyme production (Which was maximum 2311.1 U/g) with 1%. And studied on the effect of different nitrogen sources results were showed a positive impact on enzyme synthesis. In different mineral sources calcium which has result in maximum enzyme production shows (1502.2 U/g) with 0.1%. The results of present study suggest that Bacillus subtilis MAFE 118079 can be used for the alpha amylase production to cover the growing need of this enzyme in detergent industrial purposes.