Cytomegalovirus DNA quantification using an automated platform for nucleic acid extraction and real-time PCR assay setup.

نویسندگان

  • Michael Forman
  • Andy Wilson
  • Alexandra Valsamakis
چکیده

Analytical performance characteristics of the QIAsymphony RGQ system with artus cytomegalovirus (CMV) reagents were determined. Measurable range spanned 2.0 to ≥ 7.0 log(10) copies/ml. The detection limit was 23 copies/ml. Intrarun and interrun coefficients of variation were ≤ 2.1% at 3.0 and 5.0 log(10) copies/ml. In clinical specimens, RGQ values were ~0.2 log(10) copies/ml higher than those in an assay using a BioRobot M48 extraction/manual reaction setup/7500 Real-Time PCR instrument. No cross-contamination was observed.

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عنوان ژورنال:
  • Journal of clinical microbiology

دوره 49 7  شماره 

صفحات  -

تاریخ انتشار 2011