A Whole Genome Sequencing (WGS) Approach to Predict Daptomycin (DAP) Susceptibility of Enterococcus faecium
نویسندگان
چکیده
2049. Comparison of the Sepsis-2 and Sepsis-3 Definitions of Sepsis and Their Ability to Predict Mortality in a Prospective Intensive Care Unit Cohort Debra D. Poutsiaka, MD, PhD, FIDSA; Maura Porto, MD; Whitney Perry, MD; Jana Hudcova, MD; David Tybor, PhD, MPH; Susan Hadley, MD, FIDSA; Shira Doron, MD, MS, FIDSA; John Adam Reich, MD; David Snydman, MD, FIDSA and Stanley Nasraway, MD; Division of Geographic Medicine and Infectious Disease, Tufts Medical Center, Boston, Massachusetts, Department of Medicine, Tufts Medical Center, Boston, Massachusetts, Surgical Critical Care, Lahey Hospital and Medical Center, Burlington, Massachusetts, Public Health and Community Medicine, Tufts University School of Medicine, Boston, Massachusetts, Department of Anesthesiology and Perioperative Medicine, Tufts Medical Center, Boston, Massachusetts, Department of Surgery, Tufts Medical Center, Boston, Massachusetts
منابع مشابه
Whole-genome analysis of a daptomycin-susceptible enterococcus faecium strain and its daptomycin-resistant variant arising during therapy.
Development of daptomycin (DAP) resistance in Enterococcus faecalis has recently been associated with mutations in genes encoding proteins with two main functions: (i) control of the cell envelope stress response to antibiotics and antimicrobial peptides (LiaFSR system) and (ii) cell membrane phospholipid metabolism (glycerophosphoryl diester phosphodiesterase and cardiolipin synthase [cls]). H...
متن کاملWhole-genome analyses of Enterococcus faecium isolates with diverse daptomycin MICs.
Daptomycin (DAP) is a lipopeptide antibiotic frequently used as a "last-resort" antibiotic against vancomycin-resistant Enterococcus faecium (VRE). However, an important limitation for DAP therapy against VRE is the emergence of resistance during therapy. Mutations in regulatory systems involved in cell envelope homeostasis are postulated to be important mediators of DAP resistance in E. faeciu...
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Vancomycin-resistant Enterococcus faecium strains (VREfm) are critical public health concerns because they are among the leading causes of hospital-acquired bloodstream infections. Chlorhexidine (CHX) is a bisbiguanide cationic antiseptic that is routinely used for patient bathing and other infection control practices. VREfm are likely frequently exposed to CHX; however, the long-term effects o...
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Background and Objective: Enterococcal infections are considered the most common nosocomial infections. Nowadays, enterococci show high resistance to common antibiotics, especially vancomycin. Vancomycin-resistant Enterococcus faecium is one of the most common nosocomial infections, which is included in the World Health Organization priority pathogens list for research and development of new an...
متن کاملCorrelation between mutations in liaFSR of Enterococcus faecium and MIC of daptomycin: revisiting daptomycin breakpoints.
Mutations in liaFSR, a three-component regulatory system controlling cell-envelope stress response, were recently linked with the emergence of daptomycin (DAP) resistance in enterococci. Our previous work showed that a liaF mutation increased the DAP MIC of a vancomycin-resistant Enterococcus faecalis strain from 1 to 3 μg/ml (the DAP breakpoint is 4 μg/ml), suggesting that mutations in the lia...
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