Hplc Determination of Aspartame in Tabletop Sweeteners by Precolumn Derivatization Using
نویسندگان
چکیده
A new, simple normal phase high performance liquid chromatographic method was developed for the analysis of aspartame in tabletop sweeteners. The method is based on the derivatization of aspartame with 2, 4-dinitrofluorobenzene followed by detection at 332 nm. Separation was carried on silica column with hexane: ethyl acetate (60: 40, V/V) as mobile phase (pH was adjusted to 4.5 using 1% acetic acid) with a flow rate of 1 ml/min. The limit of detection (LOD) and limit of quantification (LOQ) of this method was found to be 1 ng and 4 ng respectively. The method was found to be linear in the concentration range of 20-180 ng. The recovery values of this method were found to be better than 97%.
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