Peptide Separations Using Zenix SEC-80
نویسندگان
چکیده
Size exclusion chromatography (SEC) has been widely applied in protein separation based on their molecular sizes. Here we present a peptide mixture from 1kD to 6 kD separation on size exclusion chromatography column Zenix TM -80. The effect of different mobile phases on the peptides’ separation performances was also explored. In the separation of insulin, glucagon, angiotensin I and bradykinin, 0.1% TFA/75% acetonitrile/H2O proved to be the optimum mobile phase. Peptide SEC can be applied to fractionate complex peptide mixtures as a first dimension chromatography in a multidimensional chromatography scheme. For complex E. coli tryptic digest, a mobile phase of 25 mM sodium acetate/300 mM NaCl gave the best separation.
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