Stromal Cell-Derived Factor-1α/CXCL12 Induced Chemotaxis of T Cells Involves Activation of the RasGAP-Associated Docking Protein p62Dok-1
نویسندگان
چکیده
Department of Microbiology/Immunology and the Walther Oncology Center, Indiana University School of Medicine, Indianapolis, IN 46202, and the Walther Cancer Institute, Indianapolis, IN 46208; Cancer Biology and Genetics Program, and Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, NY 10021; and First Department of Internal Medicine, Tokyo Medical University, Tokyo, Japan.
منابع مشابه
Stromal cell-derived factor-1alpha/CXCL12-induced chemotaxis of T cells involves activation of the RasGAP-associated docking protein p62Dok-1.
Events mediating stromal cell-derived factor-1 (SDF-1alpha/CXCL12) chemotaxis of lymphocytes are not completely known. We evaluated intracellular signaling through RasGAP-associated protein p62Dok-1 (downstream of tyrosine kinase [Dok-1]) and associated proteins. SDF-1alpha/CXCL12 stimulated Dok-1 tyrosine phosphorylation and association with RasGAP, adaptor protein p46Nck, and Crk-L in Jurkat ...
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A 62 kDa protein is highly phosphorylated in many cells containing activated tyrosine kinases. This protein, characterized mainly by its avid association with rasGAP, has proved elusive. Anti-phosphotyrosine antibody was used to purify p62. From peptide sequence, molecular cloning revealed a cDNA encoding a novel protein, p62dok, with little homology to others but with a prominent set of tyrosi...
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