Effect of cytokine growth factors on the prevention of acute wound failure.
نویسندگان
چکیده
Cytokine growth factor treatment of chronic wounds has met with mixed results. The chronic wound presents a hostile environment to peptides such as growth factors. Cytokine growth factors have not been studied extensively in acute wounds. However, incisional hernias are a major example of acute wound failure that has not been solved by various mechanical approaches. A biological approach to acute wound failure by use of cytokine growth factors may offer a new strategy. A rodent incisional hernia model was used. Seventy-six rats underwent 3-cm midline celiotomies and were closed with fine, fast-absorbing sutures to induce intentional acute wound failure. Group 1 received no other treatment. The midline fascia in Groups 2-10 was infiltrated with 100 microl of vehicle alone or vehicle containing various test cytokine growth factors. Necropsy was performed on postoperative day 28 and the wounds were examined for herniation. Incisional hernias developed in 83 percent (13/16) of untreated incisional and 88 percent (7/8) and 83 percent (5/6) of the two vehicle-treated incisions (PBS and carboxymethylcellulose). Hernia incidences were decreased by priming of the fascial incision with transforming growth factor-beta(2) (12%, 1/8), basic fibroblast growth factor (25%, 2/8) and interleukin-1 beta (50%, 3/6) (p < 0.05). Aqueous platelet-derived growth factor, becaplermin, insulin-like growth factor, and granulocyte macrophage-colony stimulating factor did not significantly decrease the incidence of acute wound failure (p > 0.05). A biological approach to acute wound failure as measured by incisional hernia formation can be useful in reducing the incidence of this complication. Transforming growth factor-beta(2), basic fibroblast growth factor, and interleukin 1 beta all eliminated or significantly reduced the development of incisional hernias in the rat model.
منابع مشابه
Effect of Surgical Flap on IL-1β and TGF-β Concentrations in the Gingival Crevicular Fluid of Patients with Moderate to Severe Chronic Periodontitis
Background: Growth factors play a major part in wound healing in many tissues including the periodontium. Transforming growth factor-β1 (TGF-β1) is one of these factors present in the gingival crevicular fluid. In addition, it is considered as one of the most important anti-inflammatory cytokines. Interleukin-1β is a proinflammatory cytokine that presents itself in gingival inflammation and the...
متن کاملThe Use of Amnion-Derived Cellular Cytokine Solution to Improve Healing in Acute and Chronic Wound Models
OBJECTIVE Growth factors demonstrate mixed results improving wound healing. Amnion-derived multipotent cells release physiologic levels of growth factors and tissue inhibitors of metalloproteinases. This solution was tested in models of acute and chronic wound healing. METHODS Acute model: Sprague-Dawley rats underwent laparotomy incisions. The midline fascia was primed with phosphate-buffere...
متن کاملThe Effect of Chamomile Mouthwash on the Prevention of Oral Mucositis Caused by Chemotherapy in Children with Acute Lymphoblastic Leukemia
Background: Mucositis is a complex inflammatory response of the digestive system mucous membrane and it is also one of the most common painful side effects following chemotherapy in children with carcinoma. Developing to mucositis affects the patient's life severely and can lead to long – term hospitalization.Therefore, this study was conducted to evaluate the effect of chamomile mouthwas...
متن کاملکارآیی قارچکشهای رایج در کنترل کپک خاکستری گوجهفرنگی
One of the important diseases of tomato plants is grey mold, which is caused by Botrytis cinerea. In this research, the effects of 10 fungicides on mycelial growth and conidia germination of B. cinerea were investigated. Results showed that carbendazim with a median inhibitory concentration (IC50) value of 0.1 mg/L had the highest inhibitory effect and bordeaux mixture with an IC50 value of 109...
متن کاملStudy the Effect of Bioptron Light therapy on Prevention of Oscar Production in Diabetic and Non-Diabetic Rats
Background: The formation of skin scars is inevitable following surgery, trauma and burns. Wound healing is a complex process that occurs during the competition of collagen production and collapse mechanisms. Various factors such as diabetes can interfere this process with multiple mechanisms. There are also ways to speed up the process of repair and prevent wound formation which is the use of...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society
دوره 12 1 شماره
صفحات -
تاریخ انتشار 2004