Membrane filtration of the Reiter treponeme.

Purification of TR-b, a Reiter treponeme protein antigen precipitating with antibodies in human syphilitic sera.

TR-b is a Reiter treponeme antigen, cross-reacting with an antigen in Treponema pallidum (Nichols pathogenic strain). Sera from patients with secondary syphilis contain precipitating antibodies against TR-b. The isolation of TR-b from a bacterial sonic extract is described here. It involved five fractionation steps: anion-exchange chromatography (DE-52 Whatman), gel filtration (Ac-A-22 Ultrogel...

Purification of a Reiter treponemal protein antigen that is immunologically related to an antigen in Treponema pallidum.

A protein antigen called TR-o was isolated from supernatant of a sonically treated Reiter treponeme. The isolation procedure included anion-exchange chromatography on Whatman DE-52, hydrophobic interaction chromatography on decyl agarose, and finally gel filtration on Ac-A-22 Ultrogel. The fractionations were monitored by immunoprecipitation techniques. The recovery was found to be 35%, and the...

Metabolism of Common Substrates by the Reiter Strain of Treponema pallidum.

The utilization of glucose, amino acids, fatty acids, bicarbonate, purines, and pyrimidines by the Reiter treponeme was studied by using carbon 14-labeled substrates. The distribution of carbon from the substrates into various cell components was determined. Radioactivity from labeled bicarbonate in the cellular protein was restricted to aspartic acid. The Reiter treponeme is capable of synthes...

Reiter's protein complement-fixation test; report of a trial in 1,000 unselected cases.

In recent years there has been a tendency to experiment with antigens derived from treponemata in the diagnostic tests for treponemal disease in the hope that they might prove more sensitive and more specific than the cardiolipin antigen which is now the standard preparation. These antigens have been prepared either from a virulent strain of Treponema pallidum or from the avirulent Reiter strai...

An account of the so-called Reiter treponeme (history, isolation, cultivation, specificity, and utilization).