Etio-prevalence of Sub Clinical Mastitis in Holstein X Haryana Crossbred Cattle

INTRODUCTION Mastitis is a multifaceted disease with varied etiology, damaging the dairy economy, worldwide. Hence, the importance of timely diagnosis of this disease is represented well in saying, "A day lost is quarter lost"! Focus was to investigate the etioprevalence of sub clinical mastitis (SCM) in Holstein x Haryana crossbred cattle. The relationship between somatic cell count (SCC) and cultural examination and efficiency of California mastitis test (CMT) and Chloride test in detecting SCM under field condition were also assessed. MATERIALS AND METHODS Milk samples were collected aseptically from 176 apparently healthy Holstein x Haryana crossbred (F1) cattle with no history of mastitis from organized dairy farms. Initial screening of crossbred cattle was performed by CMT and Chloride test. CMT was performed by the method of Pandit and Mehta (1969) and Chloride test by the procedure described in the laboratory manual of milk industry foundation (2005). The animals showing either CMT or Chloride test positive for a single udder was examined culturally in 5% ovine blood agar media for all the udders. The percent sensitivity, specificity were calculated by the formulae of Thrusfield (2005) and percent accuracy by the formula of Reddy et al. (2001). The identification of bacteria was done on the basis of colony characteristics, morphology and Gram's reaction (Tuteja et al., 2001). The SCC was done microscopically (Schalm et al. 1971). RESULTS AND DISCUSSION From 702 milk samples from 176 cows, 255 ETIO-PREVALENCE OF SUB CLINICAL MASTITIS IN HOLSTEIN X HARYANA CROSSBRED CATTLE Anirban Guha1 and Sandeep Gera2