A ratiometric fluorescent probe for in situ quantification of basal mitochondrial hypochlorite in cancer cells.

نویسندگان

  • Ji-Ting Hou
  • Kun Li
  • Jin Yang
  • Kang-Kang Yu
  • Ye-Xin Liao
  • Yu-Zhao Ran
  • Yan-Hong Liu
  • Xue-Dong Zhou
  • Xiao-Qi Yu
چکیده

A ratiometric fluorescent probe () for ClO(-) based on the conjugate of coumarin-rhodamine was presented, which could sense ClO(-) with fast response (within 5 s), high sensitivity and excellent selectivity. More importantly, is the first mitochondria-targeted ratiometric fluorescent probe to image exogenous and endogenous ClO(-).

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Real-time detection of hypochlorite in tap water and biological samples by a colorimetric, ratiometric and near-infrared fluorescent turn-on probe.

In this paper, we report a highly selective and sensitive ratiometric NIR fluorescent probe that can be used for real-time detection of the biologically important hypochlorite with colorimetric and significant NIR fluorescent turn-on signal changes at NIR excitation wavelength. In addition, experiments showed that this probe can be applied to detect hypochlorite in tap water, serum samples, and...

متن کامل

Development of an ICT-based ratiometric fluorescent hypochlorite probe suitable for living cell imaging.

We have judiciously constructed a novel ICT-based ratiometric OCl(-) probe capable of ratiometric imaging in the live cells based on the new OCl(-)-promoted de-diaminomaleonitrile reaction.

متن کامل

P-11: DHR123: A Suitable Fluorescent Mitochondrial Probe for Assessment of H2O2 in Human Spermatozoa

Background: The objective of present study was to assess potential of DHR123 (dihydrorhodamine 123) fluorescent probe for measurement of H2O2 produced by human spermatozoa and comparing the results with DCFH-DA (2', 7'-dichlorodihydrofluorescein diacetate). Materials and Methods: Fluorescent intensity and percentage R123 and DCF positive sperm measured by flow cytometry. The suitable ...

متن کامل

Cd(II)-terpyridine-based complex as a ratiometric fluorescent probe for pyrophosphate detection in solution and as an imaging agent in living cells.

The terpyridine anthracene ligand was synthesized and characterized. is a ratiometric fluorescent probe for Cd(2+) with a recognition mechanism based on intramolecular charge transfer (ICT). An complex was isolated, and its structure was established using single-crystal XRD. The complex was able to serve as a novel reversible chemosensing ensemble to allow ratiometric response to pyrophosphate ...

متن کامل

Fluorescent in Situ Hybridization and Real-Time Quantitative Polymerase Chain Reaction to Evaluate HER-2/neu Status in Breast Cancer

Background:Breast cancer remains the most common and second lethal cancer in females. HER-2/neu is one of the most important amplified oncogene in breast cancer. The amplification of HER-2 is correlated with decreased survival, metastasis, and early recurrence.  The amplification of HER-2/neu gene and synthesis of th...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Chemical communications

دوره 51 31  شماره 

صفحات  -

تاریخ انتشار 2015