AREGU May 45/5
نویسندگان
چکیده
Ikezaki, Hiroyuki, Manisha Patel, Hayat Önyüksel, Syed R. Akhter, Xiao-pei Gao, and Israel Rubinstein. Exogenous calmodulin potentiates vasodilation elicited by phospholipid-associated VIP in vivo. Am. J. Physiol. 276 (Regulatory Integrative Comp. Physiol. 45): R1359–R1365, 1999.—The purpose of this study was to determine whether exogenous calmodulin potentiates vasoactive intestinal peptide (VIP)-induced vasodilation in vivo and, if so, whether this response is amplified by association of VIP with sterically stabilized liposomes. Using intravital microscopy, we found that calmodulin suffused together with aqueous and liposomal VIP did not potentiate vasodilation elicited by VIP in the in situ hamster cheek pouch. However, preincubation of calmodulin with liposomal, but not aqueous, VIP for 1 and 2 h and overnight at 4°C before suffusion significantly potentiated vasodilation (P , 0.05). Calmodulin-induced responses were significantly attenuated by calmidazolium, trifluoperazine, and NG-nitro-L-arginine methyl ester (L-NAME) but not D-NAME. The effects of L-NAME were reversed by Lbut not D-arginine. Indomethacin had no significant effects on calmodulin-induced responses. Calmodulin had no significant effects on adenosine-, isoproterenol-, acetylcholine-, and calcium ionophore A-23187-induced vasodilation. Collectively, these data indicate that exogenous calmodulin amplifies vasodilation elicited by phospholipid-associated, but not aqueous, VIP in the in situ peripheral microcirculation in a specific, calmodulin active sites-, and nitric oxide-dependent fashion. We suggest that extracellular calmodulin, phospholipids, and VIP form a novel functionally coordinated class of endogenous vasodilators.
منابع مشابه
AREGU May 45/5
S. TAHERZADEH,1 S. SHARMA,1 V. CHHAJLANI,2 I. GANTZ,3 N. RAJORA,1 M. T. DEMITRI,4 L. KELLY,1 H. ZHAO,1 T. ICHIYAMA,1 A. CATANIA,4 AND J. M. LIPTON1,5 Departments of 1Physiology and 5Anesthesiology and Pain Management, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75235-9040; 2Astra Hassle, 431 83 Molndal, Sweden; 3Department of Surgery, University of Michigan Medical ...
متن کاملAREGU Mar. 45/3
M. J. P. LENCZOWSKI,1 R.-M. BLUTHÉ,2 J. ROTH,3 G. S. REES,4 D. A. RUSHFORTH,5 A.-M. VAN DAM,1 F. J. H. TILDERS,1 R. DANTZER,2 N. J. ROTHWELL,5 AND G. N. LUHESHI5 1Department of Pharmacology, Faculty of Medicine, Research Institute Neurosciences Vrije Universiteit, Graduate School Neurosciences Amsterdam, 1081 BT Amsterdam, The Netherlands; 2Institut François Magendie, Institut National de la Re...
متن کاملAREGU May 45/5
Buñag, Ruben, Jennifer Mellick, and Brandy Allen. Abated cardiovascular responses to chronic oral lisinopril treatment in conscious elderly rats. Am. J. Physiol. 276 (Regulatory Integrative Comp. Physiol. 45): R1408–R1415, 1999.—To determine whether the cardiovascular effects of chronic treatment with lisinopril are age related, we compared baroreflex sensitivity and pressor responsiveness in 4...
متن کاملAREGU May 45/5
Engeland, W. C., and B. K. Levay-Young. Changes in the glomerulosa cell phenotype during adrenal regeneration in rats. Am. J. Physiol. 276 (Regulatory Integrative Comp. Physiol. 45): R1374–R1382, 1999.—In situ hybridization was used to examine cellular differentiation during rat adrenal regeneration, defining zona glomerulosa [cytochrome P-450 aldosterone synthase (P-450aldo) mRNA positive], zo...
متن کاملAREGU May 45/5
Biesiadecki, Brandon J., Paul H. Brand, Lauren G. Koch, Patricia J. Metting, and Steven L. Britton. Phenotypic variation in sensorimotor performance among eleven inbred rat strains. Am. J. Physiol. 276 (Regulatory Integrative Comp. Physiol. 45): R1383–R1389, 1999.—As a first step toward identifying the genes that determine sensorimotor ability (motor coordination) we subjected 11 inbred strains...
متن کاملAREGU May 45/5
Woods, Robyn L., and Marcus J. M. Jones. Atrial, B-type, and C-type natriuretic peptides cause mesenteric vasoconstriction in conscious dogs. Am. J. Physiol. 276 (Regulatory Integrative Comp. Physiol. 45): R1443–R1452, 1999.— Cardiovascular responses were compared with equimolar infusions of B-type (BNP) and C-type (CNP) with atrial natriuretic peptide (ANP) in conscious, instrumented dogs. On ...
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