Resolution of racemic phenylalanine.

A Crystallization-Induced Asymmetric Transformation using Racemic Phenyl Alanine Methyl Ester Derivatives as Versatile Precursors to Prepare Amino Acids

L-Tyrosine and L-Dopa are the precursors in the biological synthesis of amine neurotransmitters. On the other hand, phenylalanine as an aromatic amino acid (AAA) is a precursor in the synthesis of L-Tyrosine and L-Dopa. For some substrates such as amino acids, resolution by the formation of diastereomers offers an attractive alternative. Among different methods in this case, crystallization-ind...

Resolution of racemic phenylalanine, tyrosine, and tryptophan.

Efficient Preparation of Enantiopure D-Phenylalanine through Asymmetric Resolution Using Immobilized Phenylalanine Ammonia-Lyase from Rhodotorula glutinis JN-1 in a Recirculating Packed-Bed Reactor

An efficient enzymatic process was developed to produce optically pure D-phenylalanine through asymmetric resolution of the racemic DL-phenylalanine using immobilized phenylalanine ammonia-lyase (RgPAL) from Rhodotorula glutinis JN-1. RgPAL was immobilized on a modified mesoporous silica support (MCM-41-NH-GA). The resulting MCM-41-NH-GA-RgPAL showed high activity and stability. The resolution ...

Mutants of Phenylalanine Dehydrogenase: New Bio-Catalysts in the Asymmetric Synthesis and Racemic Resolution of Non-Natural Aminocids

Paul C. Engel, Francesca Paradisi, Eamonn Moynihan and Anita R. Maguire, (1)Department of Biochemistry, University College Dublin, Conway Institute of Biomolecular and Biomedical Research, Belfield Campus, Dublin, 4, Ireland, (2)Department of Biochemistry, University College Dublin, Conway Institute, Belfield Campus, Dublin, 4, Ireland, (3)Department of Chemistry, University College Cork, Cork,...

Separation of Racemic Mixtures of Amino Acids Using Chiral Eluents

A commercially available non-chiral RP-C8 chromatographic column is used with an aqueous chiral mobile phase containing N,N-dimethyl-L-phenylalanine and copper in a ratio 20 : 1 for the enantiomeric resolution of the following amino acids: DL-valine (Val), DL-methionine (Met), DL-leucine (Leu), DL-phenylalanine (Phe) and DL-tyrosine (Tyr). The influence of organic modifier, temperature and flow...