Changes of plasma prothrombin, protein C and urinary Y-carboxyglutamic acid in diabetes mellitus
نویسندگان
چکیده
Plasma levels vitamin K dependent coagulation factors, for example, prothrombin and protein C and urinary r-carboxyglutamic acid (r-Gla) were measured in diabetes mellitus. Concentration of prothrombin was determined by Echis carinatus-chromogenic method. (S-2238) and protein C was determined by ELISA. Concentration of urinary r-Gla was determined using HPLC by Kuwada, et. al. Plasma samples were collected from 10 healthy normal adults, 30 patients with diabetes mellitus. The results obtained were as follows. Plasma prothrombin and protein C concentration were increased in diabetes mellitus and these two parameter were significantly correlated. Concentration of urinary r-Gla were significantly increased in diabetes mellitus. A good correlation existed between plasma prothrombin and urinary r-Gla among patients with diabetes mellitus. There was also a good correlation between plasma protein C and urinary r-Gla in patients with diabetes mellitus. These results were suggested that vitamin K dependent coagulation factors indicated increase of hepatic protein synthesis and hyperfunction of vitamine K in liver of diabetes mellitus.
منابع مشابه
Discrimination of Normal and Abnormal Prothrombin and Protein C in Plasma Using a Calcium ton-Inhibited Monoclonal Antibody to a Common Epitope on Several Vitamin K-Dependent Proteins
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