A label-free activatable aptamer probe for colorimetric detection of cancer cells based on binding-triggered in situ catalysis of split DNAzyme.

نویسندگان

  • Hui Shi
  • Duo Li
  • Fengzhou Xu
  • Xiaoxiao He
  • Kemin Wang
  • Xiaosheng Ye
  • Jinlu Tang
  • Chunmei He
چکیده

A novel label-free tailed hairpin-shaped activatable aptamer probe (THAAP) was developed by rationally integrating an aptamer and a split G-quadruplex into one sequence. Based on target recognition-triggered in situ catalysis of split DNAzyme, the THAAP strategy achieved a simple, fast, washing-free, specific and quantitative colorimetric assay of human leukemic CCRF-CEM cells.

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عنوان ژورنال:
  • The Analyst

دوره 139 17  شماره 

صفحات  -

تاریخ انتشار 2014