Urothelial Cells Increased p16 Levels Correlate with pRb Alterations in Human

The CDKN2 (MISI) gene is located at 9p21; its product, p 16. inhibits the cyclin D/CDK4 complex that phosphorylates pRb, thus negatively regulating cell cycle progression IM. Serrano et al., Nature i l.oml.i. 366: 704, 1994; A. Kamb et al, Science (Washington DC), 264: 436, 1994; T. Nobori et al.. Nature (Lond.), 368: 753,1994]. CDKN2 mutations are more common in cultured human uroepithelial cells (HUC) than in uncultured bladder cancers. We examined the status of CDKN2/pl6 in early and late passage (P) cultures of HUC. HUC immortalization was not accompanied by pl6 loss, even in cells with a hemizygous 9p21-pter deletion, but late passage cultures with a pl6 loss showed decreased generation time. Thus, the data do not indicate that CDKN2 is a candidate for a chromosome 9 senescence gene but suggest that pl6 loss may confer a growth advantage in vitro. Significant differences in pl6 levels were observed among HUC cell lines, but no CDKN2 mutations were detected. However, an inverse correlation between elevated pl6 and loss of pRb function was observed (P < 10 Y Ten samples with normal pRb showed low or undetectable p 16 levels, while seven samples with known pRb alterations showed abundant pl6 but nevertheless grew vigorously in culture. These results support the hypothesis that pl6 mediated cell cycle inhibition, as well as pl6 regulation, occurs via pRb dependent pathway(s).