Analysis of Polar Compounds Using 100% Aqueous Mobile Phases with Agilent ZORBAX Eclipse Plus Phenyl-Hexyl and Other ZORBAX Phenyl Columns
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چکیده
The analysis of polar compounds, such as acids and bases, typically requires ion pairing reagents or Hydrophobic Interaction Chromatography (HILIC) columns in HPLC. In this work, the utility of phenyl columns is demonstrated using 100% aqueous mobile phase, without the inconvenience of phase collapse. Selectivity of different Agilent ZORBAX phenyl phases (StableBond SB-Phenyl, Eclipse XDB-Phenyl and Eclipse Plus Phenyl-Hexyl) is shown with both aliphatic acids and catecholamines (bases). The selectivity of these compounds is further shown to be a function of mobile phase additive. Authors William J. Long, Anne E. Brooks, and William Biazzo Agilent Technologies, Inc. 2850 Centerville Road Wilmington, DE 19808 USA Application Note Pharmaceuticals and Food
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