Cationic Nanostructures against Foodborne Pathogens

In food microbiology, novel strategies to fight foodborne pathogens are certainly welcome. In this data report, cationic nanostructures built from combinations of nanoparticles, antimicrobial peptide and cationic lipid are evaluated against important foodborne pathogens such as Escherichia coli, Salmonella enterica subsp. serovar Typhymurium, Staphylococcus aureus and Listeria monocytogenes. The cationic lipid dioctadecyldimethylammonium bromide (DODAB), the antimicrobial peptide gramicidin D (Gr), the antimicrobial cationic polymer poly (diallyldimethylammonium chloride) (PDDA) and the biocompatible polymer poly (methyl methacrylate) (PMMA) can be combined to yield a variety of antimicrobial cationic nanostructures as previously described by our group (Carmona Ribeiro and Chaimovich, 1983; Martins et al., 1997; Lincopan et al., 2003, 2005; Pereira et al., 2008; Melo et al., 2010, 2011; Carvalho et al., 2012; Naves et al., 2013; Ragioto et al., 2014; Carrasco et al., 2015; Sanches et al., 2015). However, these nanostructures were not specifically evaluated against foodborne pathogens before. This data report aims at filling up this gap. The cationic lipid (DODAB) and the cationic polymer PDDA bear quaternary antimicrobial nitrogens and form a variety of cationic nanostructures as the closed or open bilayers; the hybrid polymeric nanoparticles NPs and the DODAB/Gr combinations. Schemes, physical properties and antimicrobial activity for the cationic assemblies against the foodborne pathogens are on the data set (https://www.researchgate.net/publication/ 308140571_September_15_2016_data_set_on_cationic_assemblies_against_food_pathogens.) DODAB, Gr, PDDA, PMMA, ethanol, 2,2,2-trifluoroethanol (TFE) and NaCl were from SigmaAldrich (St. Louis, MO, USA). DODAB LV were obtained by hydrating and vortexing the DODAB powder in 1 mM NaCl aqueous solution, at 60◦C at 2mM DODAB (Carmona Ribeiro and Chaimovich, 1983). For obtaining theDODABBF, LVwere ultrasonically disrupted with amacrotip (85W/15min/70◦C) before centrifuging (10,000 rpm/60min/4◦C) and collecting the supernatant (Carmona-Ribeiro, 2006). DODAB analysis was via microtitration of its bromide counterion (Schales and Schales, 1941). A Gr stock solution (6.4mM Gr) in TFE was added to previously prepared LV or BF at a 1:10 Gr:DODAB molar ratio. DODAB/Gr dispersions were prepared from DODAB LV incubated for 1 h/60◦C with Gr (Ragioto et al., 2014). DODAB LV/ Gr sonicated with macrotip (85W/15min/70◦C) and centrifuged (10,000 rpm/60min/4◦C) yield the DODAB BF/Gr.