CSF-1 and mouse preimplantation development in vitro.

The Evaluation of Melatonin Effect on In-Vitro Development of Mouse Preimplantation Embryos

Purpose: Melatonin promotes in-vitro embryo development in different species. This study studied the effects of melatonin on in-vitro mouse preimplantation embryo development. Materials and Methods: Two-cell embryos were obtained from oviduct of 6-8 weeks female NMRI mice 48 hours after administration of an intra-peritoneal injection of 5 IU/ml pregnant mares’ serum gonadotrophin and subsequent...

The Effect of Human Follicular Fluid (FF) on Preimplantation Mouse Embryo Development in Vitro

  SUMMARY Since at oocyte retrieval for in vitro fertilization there is some FF become incorporate to egg, we decided to investigate the effect of this fluid on preimplantation embryo development in vitro. FF was obtained at the time of oocyte retrieval for IVF, centrifuged and then either inactivated by heat and stored at 4°c or without inactivation stored at-20°c until used. Late 2-cell emb...

P-25: Study of the Effect of Experimental Diabetes Induced by STZ on In vitro Fertilization and Preimplantation Embryo Development Rate in Male Mouse Model

Background: Diabetes is a metabolic disease caused by a deficiency in the pancreatic secretion of insulin and/or by the inability of tissues to efficiently respond to insulin, events that cause hyperglycemia and affect all organs.Tissue alterations caused by diabetes affect different organic systems , including the male reproductive system. Reproductive dysfunction is one of the common secondar...

Ascorbic Acid Diminishes Developmental Damages Induced by High Glucose in Mouse Preimplantation Emberyos in vitro

The Influence of Light on Apoptosis in Preimplantation Mouse embryos in vitro

Background: In vitro culture of mammalian embryos can slow or stop growth completely. This may be due to the medium used, pH, temperature, or light. There is considerable concern about the harmful effect of light in the laboratory environment. Cell number and apoptosis are useful parameters that indicate embryonic development and health. In this study, we assessed these two factors in the blas...

Stress response genes are suppressed in mouse preimplantation embryos by granulocyte-macrophage colony-stimulating factor (GM-CSF).

BACKGROUND Granulocyte-macrophage colony-stimulating factor (GM-CSF) is known to promote the development and survival of human and mouse preimplantation embryos; however, the mechanism of action of GM-CSF in embryos is not defined. METHODS Mouse blastocysts were cultured from zygote stage in vitro with and without recombinant mouse GM-CSF (rmGM-CSF), and in vivo developed blastocysts were flu...