Pluchea lanceolata protects against Benzo(a) pyrene induced renal toxicity and loss of DNA integrity
نویسندگان
چکیده
Evidence from epidemiological, experimental and clinical trial data indicates that a plant based diet can reduce the risk of chronic diseases and reduces toxic effects. In the present study, we report the antioxidant and anticlastogenic activity of Pluchea lanceolata (PL), an important medicinal plant, in both in vitro and in vivo model. Benzo(a)pyrene (B(a)P) administration leads to depletion of renal glutathione and its metabolizing enzymes. Pretreatment with PL (100 and 200 mg /kg b.wt) restored renal glutathione content and its dependent enzymes significantly (p<0.001) with simultaneous increase in catalase(CAT), quinone reductase(QR) in mouse kidney. Prophylactic administration of PL prior to B (a) P administration significantly decreased the malondialdehyde(MDA), H2O2 and xanthineoxidase (XO) levels at a significance of p<0.001, at both the doses. PL extract pretreated groups showed marked inhibition in B(a)P induced micronuclei formation in mouse bone marrow cells with simultaneous restoration of DNA integrity, viz. alkaline unwinding assay and DNA damage shown by gel-electrophoresis. HPTLC confirms the presence of quercetin in plant extract which could be responsible for PL protecting efficacy. In conclusion, the present findings strongly support the antioxidant efficacy of PL, possibly by modulation of antioxidant armory.
منابع مشابه
Effects of Pluchea lanceolata Root Extract on Cisplatin induced Nausea and Vomiting in Rat Pica Model
Cisplatin is an effective chemotherapeutics against a wide range of cancers. However, it causes significant nausea and vomiting which limit its usefulness. In the present study, the effects of methanolic root extract of Pluchea lanceolata (DC.) C. B. Clarke , asteraceae ( MPL ) was investigated against cisplatin-induced nausea using a rat pica model. In rat pica model, rats react to cispla...
متن کاملTangeretin protects renal tubular epithelial cells against experimental cisplatin toxicity
Objective(s): Cisplatin is an effective antineoplastic agent; its clinical utility, however, is limited by a few salient toxic side effects like nephrotoxicity. This study aimed to determine the potential protective effects of tangeretin, a citrus-derived flavonoid, against renal tubular cell injury in cisplatin-induced renal toxicity of rats.Materials and Methods: Tangeretin was injected intra...
متن کاملAdaptive upregulation of DNA repair genes following benzo(a)pyrene diol epoxide protects against cell death at the expense of mutations
A coordinated and faithful DNA damage response is of central importance for maintaining genomic integrity and survival. Here, we show that exposure of human cells to benzo(a)pyrene 9,10-diol-7,8-epoxide (BPDE), the active metabolite of benzo(a)pyrene (B(a)P), which represents a most important carcinogen formed during food preparation at high temperature, smoking and by incomplete combustion pro...
متن کاملP-220: Vitamin E Protects Atrazine-Induced Toxicity in Testes of Mature Male Rats
Background: According to several reports there is a positive correlation between the lowered quality of semen and any exposure to toxic compound specially those particularly disrupt endocrine functions. Like other herbicides atrazine (ATR) induces endocrine disruption and consequently interferes with sexual hormones physiological functions. Therefore current study was designed in order to evalu...
متن کاملP-67: Nano-Selenium Protects Cisplatin-Induced Spermatotoxicty and Chromatin Abnormality Associated with Free Radicals Toxic Stress
Background: Cisplatin (CIS), an anticancer alkylating agent, induces DNA adducts and effectively cross links the DNA strands and so affects spermatozoa as a male reproductive tract toxicant. Materials and Methods: The present study investigated the cellular/biochemical mechanisms underlying possible protective effect of selenium nano-particles (Nano-Se) as an established strong antioxidant with...
متن کامل