Transmission electron microscopy enables the reconstruction of the catenane and ring forms of CS2 hydrolase.
نویسندگان
چکیده
Transmission electron microscopic studies on CS2 hydrolase provide direct evidence for the existence of the hexadecameric catenane and octameric ring topologies. Reconstructions of both protein assemblies are in good agreement with crystallographic analyses of the catenane and ring forms of CS2 hydrolase.
منابع مشابه
Evidence that the catenane form of CS2 hydrolase is not an artefact.
CS2 hydrolase, a zinc-dependent enzyme that converts carbon disulfide to carbon dioxide and hydrogen sulfide, exists as a mixture of octameric ring and hexadecameric catenane forms in solution. A combination of size exclusion chromatography, multi-angle laser light scattering, and mass spectrometric analyses revealed that the unusual catenane structure is not an artefact, but a naturally occurr...
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ورودعنوان ژورنال:
- Chemical communications
دوره 50 71 شماره
صفحات -
تاریخ انتشار 2014