Design and engineering of water-soluble light-harvesting protein maquettes† †Electronic supplementary information (ESI) available: Experimental methods and supplementary Fig. S1–10. See DOI: 10.1039/c6sc02417c Click here for additional data file.

نویسندگان

  • Goutham Kodali
  • Joshua A. Mancini
  • Lee A. Solomon
  • Tatiana V. Episova
  • Nicholas Roach
  • Christopher J. Hobbs
  • Pawel Wagner
  • Olga A. Mass
  • Kunche Aravindu
  • Jonathan E. Barnsley
  • Keith C. Gordon
  • David L. Officer
  • P. Leslie Dutton
  • Christopher C. Moser
چکیده

Natural selection in photosynthesis has engineered tetrapyrrole based, nanometer scale, light harvesting and energy capture in light-induced charge separation. By designing and creating nanometer scale artificial light harvesting and charge separating proteins, we have the opportunity to reengineer and overcome the limitations of natural selection to extend energy capture to new wavelengths and to tailor efficient systems that better meet human as opposed to cellular energetic needs. While tetrapyrrole cofactor incorporation in natural proteins is complex and often assisted by accessory proteins for cofactor transport and insertion, artificial protein functionalization relies on a practical understanding of the basic physical chemistry of protein and cofactors that drive nanometer scale self-assembly. Patterning and balancing of hydrophobic and hydrophilic tetrapyrrole substituents is critical to avoid natural or synthetic porphyrin and chlorin aggregation in aqueous media and speed cofactor partitioning into the non-polar core of a man-made water soluble protein designed according to elementary first principles of protein folding. This partitioning is followed by site-specific anchoring of tetrapyrroles to histidine ligands strategically placed for design control of rates and efficiencies of light energy and electron transfer while orienting at least one polar group towards the aqueous phase.

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منابع مشابه

A well-defined, versatile photoinitiator (salen)Co–CO2CH3 for visible light-initiated living/controlled radical polymerization† †Electronic supplementary information (ESI) available: Experimental details, characterization of (salen)Co–CO2CH3, Fig. S1–S20. CCDC 1031130. For ESI and crystallographic data in CIF or other electronic format see DOI: 10.1039/c5sc00477b Click here for additional data file. Click here for additional data file.

Yaguang Zhao,† Mengmeng Yu,† Shuailin Zhang, † Zhenqiang Wu,† Yuchu Liu,† ChiHow Peng,*‡ and Xuefeng Fu*† †Beijing National Laboratory for Molecular Sciences, State Key Lab of Rare Earth Materials Chemistry and Applications, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China ‡Department of Chemistry and Frontier Research Center on Fundamental and Applied S...

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عنوان ژورنال:

دوره 8  شماره 

صفحات  -

تاریخ انتشار 2017