Sweat induction from an isolated eccrine sweat gland.

SAW, KENZO. Sweat induction from an isolated eccrine sweat gland. Am. J. Physiol. 225(5) : 1147-l 152. 1973.-A method has been developed for in vitro induction of sweating from an isolated monkey-palm eccrine sweat gland. The validity of the new methodology is shown in the following results. MecholyLinduced sweating was completely inhibited by atropine and by cooling the incubation medium to 4 C. Ouabain inhibited both sweat secretion and sodium reabsorption. Sweat samples collected from the end of the proximal duct had a sodium concentration from 20 to 90 mM and a potassium concentration from 5 to 18 mM, whereas samples collected directly from the isolated secretory coil contained both SOdium and potassium in concentrations isotonic to the incubation medium; the results are consistent with the previous studies of others with micropuncture techniques. Epinephrine was shown to induce sweat secretion comparable in both secretory rate and SOdium and potassium concentrations to those of Mecholyl-induced sweat. An attempt was made to estimate the transport rate of the secretory coil expressedL in terms of tubular length. Volume flux from 3 to 4.7 x 10W7 cm3/cm-set thus calculated was approximately half as much as that of the rat kidney proximal tubule.