In vitro effi cacy of hyperbaric oxygen therapy against Leishmania tropica promastigotes and amastigotes
نویسندگان
چکیده
Materials and methods: Th e eff ects of HBO on Leishmania tropica promastigote and amastigote forms were studied at a pressure of 2.5 atmosphere absolute (ata). We examined the cytotoxic eff ect of HBO on promastigotes using the trypan blue exclusion test and the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) dye-reduction assay. To investigate the eff ects of HBO treatment on the growth of the promastigotes, HBO-treated and non-HBO-treated cell culture fl asks were incubated at 26 °C for 3 days, while the eff ects of HBO on L. tropica amastigotes were detected using the acridine orange staining method.
منابع مشابه
2 Carneiro.indd
The leishmaniases are severe parasitic diseases that occur worldwide, caused by protozoa of the genus Leishmania. Studies with medicinal plants can lead to a range of possibilities for treating and improving the patients’ quality of life. Research on Azadirachta indica fractions and extracts has shown that they have excellent anti-leishmanial activity based on bioactivity-guided fractionation o...
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